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KMID : 0903619900310020185
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Journal of the Korean Society for Horticultural Science
1990 Volume.31 No. 2 p.185 ~ p.192
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Induction of Genetic Transformation in Radish Plants by the Microinjection of Foreign DNA into the Ovary
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Abstract
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An experimental system was developed to transfer a genetic character by microinjecting foreign DNA into the ovary of recipient plants at the time of flowering. Total DNA extracted from young leaves of radish (Raphanus sativus L.) cv. Altari (A) was microinjected into the ovary of cv. Kungzung (K) when K was being self-pollinated. The hypocotyl color was the genetic marker to be transferred from A to K by the injection treatment. When the seeds harvested from DNA-injected K plants were grown, seven transformants were identified, the frequency of transformation being 0.25. When the seeds harvested from the transformants were regrown (TS-1 generation), the color was segregated into 3 reds to 1 white as if the TS-1 plants were obtained from crossing A and K. Results suggest that the gene controlling the color of A was transferred to K by the injection treatment and then stably incorporated into the K genome. In addition to the hypocotyl color, however, other characters were also transformed, probably because the injections were made with total DNA instead of a specific gene segment. Co-transformation of other characters resulted in a heterogeneity of all transformants. Methods tvere developed to identify the transformants at seed stage by comparing electrophoretic banding patterns of total soluble proteins and the isozymes of malate dehydrogenase and acid phosphatase. It is concluded that microinjection of foreign DNA into the ovary can be a useful way to induce genetic transformations of crop plants. Development of the methods to identify the transformants at seed stage should help to expedite the selection process involved in transformation studies.
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